Bactericidal Activity of Extracts and Major Fractions of Endophyte Metabolites of Fungus Cladosporium Velox against Pneumonia and Diarrhea Pathogens

Abstract

The resistance of bacteria to drugs is a problem in the treatment of diarrhea and pneumonia. A new source of antibiotic with high sensitivity and potential to develop as new drugs is required. This study was conducted to determine the fungus of Cladosporium and test the crude extract and the major fraction on the bacteria that cause pneumoniae and the diarrhea. Firstly, the fungus was determined its species with molecular identification. The method for bacterial sensitivity tests was carried out by disc diffusion; MIC and MBC analysis using liquid dilution; and the data were statistically analyzed using One Way ANOVA. The species was identification as Cladosporium velox shows the inhibition ranging from 29.9-38.84 mm; all MBC/MIC values <2 indicate the extract can kill all the bacteria tested. These results indicate that the antibacterial metabolites of C. velox are bactericidal and have the potential to be developed as candidates for anti-pneumonia and anti-diarrheal drugs.